| bcr-abl
Translocation for Chronic Myelogenous Leukemia
Synonyms:
Philadelphia Chromosome
Specimen:
Whole
blood or bone marrow
Volume:
7 mL whole
blood or 1-2 mL bone marrow
Minimum Volume:
3
mL whole blood
Container:
Lavender-stopper
(EDTA) tubes
Collection:
Submit
at room temperature. Specimens should arrive in the laboratory
within 48 hours of collection. Indicate date
and time of collection on test request form.
Storage:
Maintain specimen at room
temperature.
Causes for Rejection:
Specimen frozen; specimen in
formalin or other fixative; clotted specimen;
hemolysis; blood more than 72 hours old; bone
marrow aspirates older than 5 days; bags or
bottles of body fluid or bronchial washings
Use:
Aid in the
diagnosis of chronic myelogenous leukemia
(CML) and monitoring for minimal residual
disease. CML is associated with a bcr-abl
chromosomal translocation t(9; 22) in >95%
of all cases.
Methodology:
Reverse
transcriptase polymerase chain reaction
(RT-PCR)
and allele-specific oligonucleotide (ASO)
analysis
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